Methylation
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| Strain | Dam methylation | Dam gene | Dcm methylation | Dcm gene | Restriction endonuclease | Recognition motif | Wadjet | Notes | References |
|---|---|---|---|---|---|---|---|---|---|
| C. acetobutylicum | Negative | Negative | Negative | Negative | Cac824I | 5'-GCNGC-3' | [1], [2] | ||
| C. pasteurianum | Postive | Negative | Negative | Negative | CpaAI | 5’-CGCG-3’ | [3] | ||
| C.ljungdahlii | |||||||||
- ↑ In vivo methylation in Escherichia coli by the Bacillus subtilis phage phi 3T I methyltransferase to protect plasmids from restriction upon transformation of Clostridium acetobutylicum ATCC 824 - PMC
- ↑ Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum - PMC
- ↑ Development of an electrotransformation protocol for genetic manipulation of Clostridium pasteurianum | Biotechnology for Biofuels and Bioproducts | Springer Nature Link