YTF Medium. [1]
YTF Medium Composition (per liter)
| fructose
|
10 g
|
| yeast extract
|
10 g
|
| tryptone
|
16 g
|
| sodium chloride
|
0.2 g
|
| Acid 1000x trace element solution
|
1 ml
|
| Basic 1000x trace element solution
|
1 ml
|
| Vitamin 1000x stock solution
|
1 ml
|
*pH set to 5.8 using concentrated HCl
YTF Media Acid 1000x Trace element solution
| HCl
|
50 mM
|
| H3BO3
|
100 mg/L
|
| MnCl2.4H2O
|
230 mg/L
|
| FeCl2.4H2O
|
78 mg/L
|
| CoCl2.6H2O
|
103 mg/L
|
| NiCl2.6H2O
|
602 mg/L
|
| ZnCl2
|
78 mg/L
|
| CuSO4.5H2O
|
50 mg/L
|
| AlK(SO4)2.12H2O
|
50 mg/L
|
YTF Media 1000x basic trace element solution
| NaOH
|
10 mM
|
| Na2SeO3
|
58 mg/L
|
| Na2WO4
|
53 mg/L
|
| Na2MbO4.2H2O
|
52 mg/L
|
YTF Media Vitamin stock solution (mg/L)
| p-aminobenzoate
|
114
|
| Riboflavin
|
104
|
| Thiamine
|
200
|
| Nicotinate
|
206
|
| Pyridoxin
|
510
|
| Pantothenate
|
104
|
| Cyanocobalamin
|
78
|
| d-biotin
|
22
|
| Folate
|
48
|
| Lipoate/thiotic acid
|
50
|
YTF Agar Composition (per liter)
| fructose
|
10 g
|
| yeast extract
|
10 g
|
| tryptone
|
16 g
|
| sodium chloride
|
0.2 g
|
| bacteriological agar (Oxoid)
|
15 g
|
*pH 5.8, add antibiotics as needed[2]
- ↑ Woods, C., Humphreys, C. M., Tomi-Andrino, C., Henstra, A. M., Köpke, M., Simpson, S. D., Winzer, K., & Minton, N. P. (2022). Required Gene Set for Autotrophic Growth of Clostridium autoethanogenum. Applied and Environmental Microbiology, 88(7). https://doi.org/10.1128/aem.02479-21
- ↑ Liew FHenstra AM, Winzer K, Köpke M, Simpson SD, Minton NP.2016.Insights into CO2 Fixation Pathway of Clostridium autoethanogenum by Targeted Mutagenesis. mBio7:10.1128/mbio.00427-16.https://doi.org/10.1128/mbio.00427-16